New Terbium Complex for Detection of Gemifloxacin Mesylate in different Samples Using Luminescence and Time Resolved Method

Goda, Rania M.; Anwar, Zeiab; Duerkop, Axel; Mostafa, Gasser Mohamed

doi:10.21608/AELS.2023.205300.1032

New Terbium Complex for Detection of Gemifloxacin Mesylate in different Samples Using Luminescence and Time Resolved Method

Document Type : Original research articles

Authors

¹ Chemistry Department, Faculty of Science, Suez Canal University, 41522 Ismailia, Egypt

² Institute of Analytical Chemistry, Chemo and Biosensors, Faculty of Chemistry and Pharmacy, University of Regensburg, 93053 Regensburg, Germany

10.21608/AELS.2023.205300.1032

Abstract

A new optical sensing microplate was developed for rapidly determining Gemifloxacin Mesylate in its pure form, pharmaceutical preparations, and human plasma using new Tb(III)-complex with 3-allyl-2-hydroxybenzohydrazide (AZ) as a probe. The interaction of Gemifloxacin mesylate with the Tb (III)- (AZ)2 complex is based on the luminescence enhancement. The metal to ligand ratio of the complex is 1:2 as determined by a Job plot method. The calibration graph was linear over the range of 0.15–10 µg/mL, with a limit of quantification (LOQ) of 0.15 µg/mL and a detection limit (LOD) of 0.05 µg/mL. The thermodynamic analysis for the interaction GMF with Tb (III)–(AZ)2 complex was evaluated. The thermodynamic analysis showed that the interaction between the Tb (III)–(AZ)2 complex and GMF was spontaneous with negative ∆G. The established approach has been used successfully to the determination of drugs in pharmaceutical formulations and spiked plasma samples with good recovery percent and RSD less than five percent.

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